TruCulture
HOT Screen customizes and manufactures TruCulture® Systems
The development and how TruCulture® is performed
Manfred Schmolz, founder of HOT Screen invented TruCulture®. Today, HOT Screen is the contract manufacturer and developer of customized TruCulture®Systems for new applications for Rules-Based Medicine. TruCulture®is an integrated blood collection and whole blood culture system. No special skills beyond those required to draw blood are necessary to perform clinical TruCulture® tests! The test kit includes syringes containing the stimulant as well as a proprietary culture medium. Only 1 ml of blood has to be drawn into the TruCulture syringe-tube. Thereafter the blood/medium mixture is incubated in a small and simple dry-block thermostat at 37 °C. At the end of incubation (usually for 24h) the clear supernatant of the culture is separated from the cells without centrifugation, by simply inserting a valve-septum into the TruCulture tube.
Application
There are two methods (vacuum & aspiration method) to use TruCulture®. Please see the instructional video for more information.
Sample Storage & Analysis
The syringes can be stored at -20 °C and sent later to a lab where cytokines secreted into the culture fluid can be quantified. The TruCulture® system can also be easily combined with the Multi-Analyte Profiling (MAP) services of Rules-Based Medicine.
Robust and reliable data for your study
TruCulture® has been used very successfully for several years to generate research data. Many of these have been published in prestigious peer-reviewed journals. Read a short summary of the seeding paper below or the full text published in the scientific journal "Immunity".
Functional Analysis via Standardized Whole-Blood Stimulation Systems Defines the Boundaries of a Healthy Immune Response to Complex Stimuli (2014)
Summary
"Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing."
Source: Duffy D, Rouilly V, Libri V, Hasan M, Beitz B, David M, Urrutia A, Bisiaux A, Labrie ST, Dubois A, Boneca IG, Delval C, Thomas S, Rogge L, Schmolz M, Quintana-Murci L, Albert ML; Milieu Intérieur Consortium. Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli. Immunity. 2014 Mar 20;40(3):436-50. doi: 10.1016/j.immuni.2014.03.002. PMID: 24656047.
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